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anti-human p-tfeb s122  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti-human p-tfeb s122
    Anti Human P Tfeb S122, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-human p-tfeb s122/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    anti-human p-tfeb s122 - by Bioz Stars, 2026-03
    90/100 stars

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    p tfeb s122  (Cell Signaling Technology Inc)
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    Figure 2. <t>TFEB</t> knockdown reduces TNBC tumor growth and self-renewal in vivo. (A) Average tumor growth curves of HCC1806 cells transduced with either scramble (n = 10) or TFEB shRNA (n = 10) and injected orthotopically into nude mice (t test, *P < 0.05). (B) Tumor-limiting dilution assay of HCC1806 cells transduced with scramble or TFEB shRNA. NSG mice were orthotopically injected with 5 × 105
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    Figure 2. TFEB knockdown reduces TNBC tumor growth and self-renewal in vivo. (A) Average tumor growth curves of HCC1806 cells transduced with either scramble (n = 10) or TFEB shRNA (n = 10) and injected orthotopically into nude mice (t test, *P < 0.05). (B) Tumor-limiting dilution assay of HCC1806 cells transduced with scramble or TFEB shRNA. NSG mice were orthotopically injected with 5 × 105

    Journal: Life science alliance

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.

    doi: 10.26508/lsa.202302259

    Figure Lengend Snippet: Figure 2. TFEB knockdown reduces TNBC tumor growth and self-renewal in vivo. (A) Average tumor growth curves of HCC1806 cells transduced with either scramble (n = 10) or TFEB shRNA (n = 10) and injected orthotopically into nude mice (t test, *P < 0.05). (B) Tumor-limiting dilution assay of HCC1806 cells transduced with scramble or TFEB shRNA. NSG mice were orthotopically injected with 5 × 105

    Article Snippet: Antibodies against TFEB (4240), p-TFEB (S122) (87932), p-TFEB (S211) (37681), 4E-BP1 (9644), p-4E-BP1 (S65) (9451), p-4E-BP1 (T37/46) (2855), p-Rictor (2114), p-Rictor (T1135) (3806), p-ACC (3661), ACC (3676), AKT (2920), p-AKT (S473) (4060), LC3 (12741), p62 (SQSTM1) (88588), Ero1-Lα (3264), BiP (3177), IRE1α (3294), PDI (3501), CHOP (2895), PERK (5683), MYC (2276), GAPDH (5174), histone H3 (14269), and lamin A/C (4777) were purchased from Cell Signaling Technology.

    Techniques: Knockdown, In Vivo, Transduction, shRNA, Injection, Limiting Dilution Assay

    Figure 6. BiP/HSPA5 knockdown suppresses TNBC self-renewal. (A) Western blot of indicated UPR markers in either scramble or BiP/HSPA5 KD cells treated with either vehicle or 2-DG for 24 h. (B, C) Clonogenic assay and (C) mammosphere formation assay of indicated cell lines transduced with either scramble or BiP/HSPA5 shRNA. Scale bar: 200 μm. (D) Flow cytometric analysis of CD44high/ CD24low cells in HCC1806 transduced with either scramble or TFEB shRNA (t test, *P < 0.05). Veh, vehicle.

    Journal: Life science alliance

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.

    doi: 10.26508/lsa.202302259

    Figure Lengend Snippet: Figure 6. BiP/HSPA5 knockdown suppresses TNBC self-renewal. (A) Western blot of indicated UPR markers in either scramble or BiP/HSPA5 KD cells treated with either vehicle or 2-DG for 24 h. (B, C) Clonogenic assay and (C) mammosphere formation assay of indicated cell lines transduced with either scramble or BiP/HSPA5 shRNA. Scale bar: 200 μm. (D) Flow cytometric analysis of CD44high/ CD24low cells in HCC1806 transduced with either scramble or TFEB shRNA (t test, *P < 0.05). Veh, vehicle.

    Article Snippet: Antibodies against TFEB (4240), p-TFEB (S122) (87932), p-TFEB (S211) (37681), 4E-BP1 (9644), p-4E-BP1 (S65) (9451), p-4E-BP1 (T37/46) (2855), p-Rictor (2114), p-Rictor (T1135) (3806), p-ACC (3661), ACC (3676), AKT (2920), p-AKT (S473) (4060), LC3 (12741), p62 (SQSTM1) (88588), Ero1-Lα (3264), BiP (3177), IRE1α (3294), PDI (3501), CHOP (2895), PERK (5683), MYC (2276), GAPDH (5174), histone H3 (14269), and lamin A/C (4777) were purchased from Cell Signaling Technology.

    Techniques: Knockdown, Western Blot, Clonogenic Assay, Tube Formation Assay, Transduction, shRNA

    Figure 7. TFEB induces autophagy in response to 2-DG. (A) Western blot of p62/SQSTM1 and LC3 in either scramble or TFEB KD treated with either vehicle or 2-DG for 24 h. (B) Western blot of LC3, p62/ SQSTM1, and BiP in cells treated with vehicle, 2- DG, and/or ISRIB for 24 h. (C) Representative ICC images of LC3 and DAPI in HCC1806 cells treated with vehicle, 2-DG, ISRIB, 2-DG + ISRIB, or hydroxychloroquine (one-way ANOVA, Dunnett’s test). Scale bar: 25 μm. Veh, vehicle; HXQ, hydroxychloroquine. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: Life science alliance

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.

    doi: 10.26508/lsa.202302259

    Figure Lengend Snippet: Figure 7. TFEB induces autophagy in response to 2-DG. (A) Western blot of p62/SQSTM1 and LC3 in either scramble or TFEB KD treated with either vehicle or 2-DG for 24 h. (B) Western blot of LC3, p62/ SQSTM1, and BiP in cells treated with vehicle, 2- DG, and/or ISRIB for 24 h. (C) Representative ICC images of LC3 and DAPI in HCC1806 cells treated with vehicle, 2-DG, ISRIB, 2-DG + ISRIB, or hydroxychloroquine (one-way ANOVA, Dunnett’s test). Scale bar: 25 μm. Veh, vehicle; HXQ, hydroxychloroquine. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: Antibodies against TFEB (4240), p-TFEB (S122) (87932), p-TFEB (S211) (37681), 4E-BP1 (9644), p-4E-BP1 (S65) (9451), p-4E-BP1 (T37/46) (2855), p-Rictor (2114), p-Rictor (T1135) (3806), p-ACC (3661), ACC (3676), AKT (2920), p-AKT (S473) (4060), LC3 (12741), p62 (SQSTM1) (88588), Ero1-Lα (3264), BiP (3177), IRE1α (3294), PDI (3501), CHOP (2895), PERK (5683), MYC (2276), GAPDH (5174), histone H3 (14269), and lamin A/C (4777) were purchased from Cell Signaling Technology.

    Techniques: Western Blot